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Paraffin Embedding Contributes to RNA Aggregation, Reduced RNA Yield, and Low RNA Quality
The RNA isolated from FFPE tissues is of poor quality and quantity. Other studies have indicated that formaldehyde fixation or the duration of storage of tissue blocks accounted for RNA damage. Herein we report a third source of harm to RNA: embedding in warm paraffin. RNA bound to oligo(dT)-conjuga...
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| Autori principali: | , , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
American Society for Investigative Pathology
2011
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3194049/ https://ncbi.nlm.nih.gov/pubmed/21884819 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmoldx.2011.06.007 |
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