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Elastin-like Polypeptides as a Purification Tag for Recombinant Proteins

This unit presents a recombinant protein purification method that employs an Elastin-like polypeptide (ELP) as a purification tag. ELPs undergo a sharp and reversible phase transition when heated above their lower critical solution temperature (LCST). ELPs retain this behavior when they are fused to...

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書目詳細資料
Main Authors: Hassouneh, Wafa, Christensen, Trine, Chilkoti, Ashutosh
格式: Artigo
語言:Inglês
出版: 2010
主題:
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3076942/
https://ncbi.nlm.nih.gov/pubmed/20814933
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/0471140864.ps0611s61
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