Knocking out multi-gene redundancies via cycles of sexual assortment and fluorescence selection

Phenotypes that might otherwise reveal a gene’s function can be obscured by genes with overlapping function. This phenomenon is best-known within gene families, where an important shared function may only be revealed by mutating all family members. Here we describe the ‘Green Monster’ technology ena...

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Detalhes bibliográficos
Main Authors: Suzuki, Yo, St. Onge, Robert P., Mani, Ramamurthy, King, Oliver D., Heilbut, Adrian, Labunskyy, Vyacheslav M., Chen, Weidong, Pham, Linda, Zhang, Lan V., Tong, Amy H. Y., Nislow, Corey, Giaever, Guri, Gladyshev, Vadim N., Vidal, Marc, Schow, Peter, Lehár, Joseph, Roth, Frederick P.
Formato: Artigo
Idioma:Inglês
Publicado em: 2011
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3076670/
https://ncbi.nlm.nih.gov/pubmed/21217751
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1550
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