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Generating Knock-in Parasites: Integration of an Ornithine Decarboxylase Transgene into its Chromosomal Locus in Leishmania donovani

Leishmania null mutants created by targeted gene replacement are typically complemented with chimeric episomes harboring the replaced gene in order to validate that the observed phenotype is due to the specific gene deletion. However, the current inventory of available episomes for complementation o...

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Autori principali: Roberts, Sigrid C., Kline, Chelsey, Liu, Wei, Ullman, Buddy
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2011
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3076542/
https://ncbi.nlm.nih.gov/pubmed/21354142
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.exppara.2011.02.020
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