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Generating Knock-in Parasites: Integration of an Ornithine Decarboxylase Transgene into its Chromosomal Locus in Leishmania donovani

Leishmania null mutants created by targeted gene replacement are typically complemented with chimeric episomes harboring the replaced gene in order to validate that the observed phenotype is due to the specific gene deletion. However, the current inventory of available episomes for complementation o...

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Autores principales: Roberts, Sigrid C., Kline, Chelsey, Liu, Wei, Ullman, Buddy
Formato: Artigo
Lenguaje:Inglês
Publicado: 2011
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3076542/
https://ncbi.nlm.nih.gov/pubmed/21354142
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.exppara.2011.02.020
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