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Enhanced levels of λ Red-mediated recombinants in mismatch repair mutants

Homologous recombination can be used to generate recombinants on episomes or directly on the Escherichia coli chromosome with PCR products or synthetic single-stranded DNA (ssDNA) oligonucleotides (oligos). Such recombination is possible because bacteriophage λ-encoded functions, called Red, efficie...

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Autors principals: Costantino, Nina, Court, Donald L.
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 2003
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC307639/
https://ncbi.nlm.nih.gov/pubmed/14673109
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.2434959100
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