Enhanced levels of λ Red-mediated recombinants in mismatch repair mutants
Homologous recombination can be used to generate recombinants on episomes or directly on the Escherichia coli chromosome with PCR products or synthetic single-stranded DNA (ssDNA) oligonucleotides (oligos). Such recombination is possible because bacteriophage λ-encoded functions, called Red, efficie...
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| Published in: | Proc Natl Acad Sci U S A |
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| Main Authors: | , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
National Academy of Sciences
2003
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC307639/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/14673109/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.2434959100 |
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