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Enhanced levels of λ Red-mediated recombinants in mismatch repair mutants
Homologous recombination can be used to generate recombinants on episomes or directly on the Escherichia coli chromosome with PCR products or synthetic single-stranded DNA (ssDNA) oligonucleotides (oligos). Such recombination is possible because bacteriophage λ-encoded functions, called Red, efficie...
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| 主要な著者: | , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
National Academy of Sciences
2003
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC307639/ https://ncbi.nlm.nih.gov/pubmed/14673109 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.2434959100 |
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