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Multicopy Plasmid Modification with Phage λ Red Recombineering

Recombineering, in vivo genetic engineering using the bacteriophage λ Red generalized recombination system, was used to create various modifications of a multicopy plasmid derived from pBR322. All genetic modifications possible on the E. coli chromosome and on bacterial artificial chromosomes (BACs)...

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Bibliografske podrobnosti
Main Authors: Thomason, Lynn C., Costantino, Nina, Shaw, Dana V., Court, Donald L.
Format: Artigo
Jezik:Inglês
Izdano: 2007
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2706537/
https://ncbi.nlm.nih.gov/pubmed/17434584
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.plasmid.2007.03.001
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