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Multicopy Plasmid Modification with Phage λ Red Recombineering
Recombineering, in vivo genetic engineering using the bacteriophage λ Red generalized recombination system, was used to create various modifications of a multicopy plasmid derived from pBR322. All genetic modifications possible on the E. coli chromosome and on bacterial artificial chromosomes (BACs)...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2007
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2706537/ https://ncbi.nlm.nih.gov/pubmed/17434584 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.plasmid.2007.03.001 |
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