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A broadly applicable continuous spectrophotometric assay for measuring aminoacyl-tRNA synthetase activity.
We describe a convenient, simple and novel continuous spectrophotometric method for the determination of aminoacyl-tRNA synthetase activity. The assay relies upon the measurement of inorganic pyrophosphate generated in the first step of the aminoacylation of a tRNA. Pyrophosphate release is coupled...
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| Auteurs principaux: | , , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
1995
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| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC307126/ https://ncbi.nlm.nih.gov/pubmed/7659511 |
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