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A broadly applicable continuous spectrophotometric assay for measuring aminoacyl-tRNA synthetase activity.

We describe a convenient, simple and novel continuous spectrophotometric method for the determination of aminoacyl-tRNA synthetase activity. The assay relies upon the measurement of inorganic pyrophosphate generated in the first step of the aminoacylation of a tRNA. Pyrophosphate release is coupled...

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Bibliografiska uppgifter
Huvudupphovsmän: Lloyd, A J, Thomann, H U, Ibba, M, Söll, D
Materialtyp: Artigo
Språk:Inglês
Publicerad: 1995
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC307126/
https://ncbi.nlm.nih.gov/pubmed/7659511
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