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A broadly applicable continuous spectrophotometric assay for measuring aminoacyl-tRNA synthetase activity.

We describe a convenient, simple and novel continuous spectrophotometric method for the determination of aminoacyl-tRNA synthetase activity. The assay relies upon the measurement of inorganic pyrophosphate generated in the first step of the aminoacylation of a tRNA. Pyrophosphate release is coupled...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Lloyd, A J, Thomann, H U, Ibba, M, Söll, D
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 1995
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC307126/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7659511/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/23.15.2886
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