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A broadly applicable continuous spectrophotometric assay for measuring aminoacyl-tRNA synthetase activity.
We describe a convenient, simple and novel continuous spectrophotometric method for the determination of aminoacyl-tRNA synthetase activity. The assay relies upon the measurement of inorganic pyrophosphate generated in the first step of the aminoacylation of a tRNA. Pyrophosphate release is coupled...
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| Veröffentlicht in: | Nucleic Acids Res |
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| Hauptverfasser: | , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
1995
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| Online Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC307126/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7659511/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/23.15.2886 |
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