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A Rapid Procedure to Purify E. coli DNA Topoisomerase I

On the basis of the asymmetrical charge distribution of E. coli DNA topoisomerase I, we developed a new procedure to purify E. coli DNA topoismoerase I in the milligram range. The new procedure includes using both cation- and anion-exchange columns, i.e., SP-Sepharose FF and Q-Sepharose FF columns....

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Bibliografski detalji
Glavni autori: Xu, Xiaozhou, Leng, Fenfei
Format: Artigo
Jezik:Inglês
Izdano: 2011
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3070380/
https://ncbi.nlm.nih.gov/pubmed/21310243
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2011.02.002
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