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A method for soluble overexpression of the α7 nicotinic acetylcholine receptor extracellular domain
We describe the construction of a soluble protein carrying the N-terminal extracellular domain (ECD) of the α7 subunit of the nicotinic acetylcholine receptor. The approach was to fuse the α7 ECD at the C and N termini of several monomeric and pentameric soluble carrier proteins and to investigate t...
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| Hauptverfasser: | , , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
The National Academy of Sciences
2001
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC30693/ https://ncbi.nlm.nih.gov/pubmed/11248118 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.041594798 |
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