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A method for soluble overexpression of the α7 nicotinic acetylcholine receptor extracellular domain

We describe the construction of a soluble protein carrying the N-terminal extracellular domain (ECD) of the α7 subunit of the nicotinic acetylcholine receptor. The approach was to fuse the α7 ECD at the C and N termini of several monomeric and pentameric soluble carrier proteins and to investigate t...

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Bibliografische gegevens
Hoofdauteurs: Fischer, Markus, Corringer, Pierre-Jean, Schott, Karin, Bacher, Adelbert, Changeux, Jean-Pierre
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: The National Academy of Sciences 2001
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC30693/
https://ncbi.nlm.nih.gov/pubmed/11248118
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.041594798
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