Detection of platinum-DNA adducts by 32P-postlabelling.
We developed a sensitive 32P-postlabeling method for the detection of bifunctional intrastrand crosslinks d(Pt-GpG) and d(Pt-ApG) in DNA in vitro and in vivo. After enzymatic digestion of DNA the positively charged platinum adducts were purified from unplatinated products, using strong cation exchan...
Guardat en:
| Publicat a: | Nucleic Acids Res |
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| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
1995
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| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC306853/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7753620/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/23.8.1300 |
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