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Detection of platinum-DNA adducts by 32P-postlabelling.

We developed a sensitive 32P-postlabeling method for the detection of bifunctional intrastrand crosslinks d(Pt-GpG) and d(Pt-ApG) in DNA in vitro and in vivo. After enzymatic digestion of DNA the positively charged platinum adducts were purified from unplatinated products, using strong cation exchan...

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Detalles Bibliográficos
Autores principales: Blommaert, F A, Saris, C P
Formato: Artigo
Lenguaje:Inglês
Publicado: 1995
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC306853/
https://ncbi.nlm.nih.gov/pubmed/7753620
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