Tyrosine 27 of the specificity polypeptide of EcoKI can be UV crosslinked to a bromodeoxyuridine-substituted DNA target sequence.
The specificity (S) subunit of the restriction enzyme EcoKI imparts specificity for the sequence AAC(N6)GTGC. Substitution of thymine with bromodeoxyuridine in a 25 bp DNA duplex containing this sequence stimulated UV light-induced covalent crosslinking to the S subunit. Crosslinking occurred only a...
Na minha lista:
| 發表在: | Nucleic Acids Res |
|---|---|
| Principais autores: | , , , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Oxford University Press
1995
|
| 在線閱讀: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC306828/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7739896/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/23.7.1177 |
| 標簽: |
沒有標簽, 成為第一個標記此記錄!
|