Tyrosine 27 of the specificity polypeptide of EcoKI can be UV crosslinked to a bromodeoxyuridine-substituted DNA target sequence.

The specificity (S) subunit of the restriction enzyme EcoKI imparts specificity for the sequence AAC(N6)GTGC. Substitution of thymine with bromodeoxyuridine in a 25 bp DNA duplex containing this sequence stimulated UV light-induced covalent crosslinking to the S subunit. Crosslinking occurred only a...

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Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	Chen, A, Powell, L M, Dryden, D T, Murray, N E, Brown, T
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 1995
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC306828/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7739896/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/23.7.1177
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