Tyrosine 27 of the specificity polypeptide of EcoKI can be UV crosslinked to a bromodeoxyuridine-substituted DNA target sequence.

The specificity (S) subunit of the restriction enzyme EcoKI imparts specificity for the sequence AAC(N6)GTGC. Substitution of thymine with bromodeoxyuridine in a 25 bp DNA duplex containing this sequence stimulated UV light-induced covalent crosslinking to the S subunit. Crosslinking occurred only a...

ver descrição completa

Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Chen, A, Powell, L M, Dryden, D T, Murray, N E, Brown, T
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 1995
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC306828/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7739896/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/23.7.1177
Tags:	Adicionar Tag Sem tags, seja o primeiro a adicionar uma tag!