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Detection and identification of 4-hydroxy-2-nonenal Schiff-base adducts along with products of Michael addition using data-dependent neutral loss-driven MS(3) acquisition: method evaluation through an in vitro study on cytochrome c oxidase modifications

We report a data-dependent neutral-loss driven MS(3) acquisition to enhance, in addition to abundant Michael adducts, the detection and localization of Schiff-base adducts of proteins and 4-hydroxy-2-nonenal, a reactive end-products of lipid peroxidation. In vitro modification of cytochrome c oxidas...

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Detalhes bibliográficos
Main Authors: Rauniyar, Navin, Prokai, Laszlo
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3065305/
https://ncbi.nlm.nih.gov/pubmed/19771555
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pmic.200900116
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