Ligand-independent assembly of recombinant human CD1 by using oxidative refolding chromatography

CD1 is an MHC class I-like antigen-presenting molecule consisting of a heavy chain and β(2)-microglobulin light chain. The in vitro refolding of synthetic MHC class I molecules has always required the presence of ligand. We report here the use of a folding method using an immobilized chaperone fragm...

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Detalhes bibliográficos
Main Authors: Altamirano, Myriam M., Woolfson, Adrian, Donda, Alena, Shamshiev, Abdijapar, Briseño-Roa, Luis, Foster, Nicholas W., Veprintsev, Dmitry B., De Libero, Gennaro, Fersht, Alan R., Milstein, César
Formato: Artigo
Idioma:Inglês
Publicado em: The National Academy of Sciences 2001
Assuntos:
Biological Sciences
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC30646/
https://ncbi.nlm.nih.gov/pubmed/11248071
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.041596598
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