Refolding chromatography with immobilized mini-chaperones

Mini-chaperones (e.g., a peptide consisting of residues 191-345 of GroEL) that are immobilized on agarose have very efficient chaperoning activity with several proteins that are otherwise recalcitrant to renaturation by conventional methods. We have used immobilized mini-chaperones both in column ch...

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Main Authors: Altamirano, Myriam M., Golbik, Ralph, Zahn, Ralph, Buckle, Ashley M., Fersht, Alan R.
Format: Artigo
Sprog:Inglês
Udgivet: The National Academy of Sciences of the USA 1997
Fag:
Biological Sciences
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC20481/
https://ncbi.nlm.nih.gov/pubmed/9108018
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