Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O

The pore-forming toxin streptolysin O (SLO) can be used to reversibly permeabilize adherent and nonadherent cells, allowing delivery of molecules with up to 100 kDa mass to the cytosol. Using FITC-labeled albumin, 10(5)–10(6) molecules were estimated to be entrapped per cell. Repair of toxin lesions...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Walev, Iwan, Bhakdi, Sebastian Chakrit, Hofmann, Fred, Djonder, Nabil, Valeva, Angela, Aktories, Klaus, Bhakdi, Sucharit
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 2001
Assuntos:	Biological Sciences
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC30628/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11248053/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.051429498
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