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Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O

The pore-forming toxin streptolysin O (SLO) can be used to reversibly permeabilize adherent and nonadherent cells, allowing delivery of molecules with up to 100 kDa mass to the cytosol. Using FITC-labeled albumin, 10(5)–10(6) molecules were estimated to be entrapped per cell. Repair of toxin lesions...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Walev, Iwan, Bhakdi, Sebastian Chakrit, Hofmann, Fred, Djonder, Nabil, Valeva, Angela, Aktories, Klaus, Bhakdi, Sucharit
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 2001
Fag:
Biological Sciences
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC30628/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11248053/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.051429498
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