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Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O
The pore-forming toxin streptolysin O (SLO) can be used to reversibly permeabilize adherent and nonadherent cells, allowing delivery of molecules with up to 100 kDa mass to the cytosol. Using FITC-labeled albumin, 10(5)–10(6) molecules were estimated to be entrapped per cell. Repair of toxin lesions...
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| Hoofdauteurs: | , , , , , , |
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| Formaat: | Artigo |
| Taal: | Inglês |
| Gepubliceerd in: |
The National Academy of Sciences
2001
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| Onderwerpen: | |
| Online toegang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC30628/ https://ncbi.nlm.nih.gov/pubmed/11248053 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.051429498 |
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