Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O

The pore-forming toxin streptolysin O (SLO) can be used to reversibly permeabilize adherent and nonadherent cells, allowing delivery of molecules with up to 100 kDa mass to the cytosol. Using FITC-labeled albumin, 10(5)–10(6) molecules were estimated to be entrapped per cell. Repair of toxin lesions...

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Detalles Bibliográficos
Main Authors: Walev, Iwan, Bhakdi, Sebastian Chakrit, Hofmann, Fred, Djonder, Nabil, Valeva, Angela, Aktories, Klaus, Bhakdi, Sucharit
Formato: Artigo
Idioma:Inglês
Publicado: The National Academy of Sciences 2001
Assuntos:
Biological Sciences
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC30628/
https://ncbi.nlm.nih.gov/pubmed/11248053
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.051429498
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