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Functional study of the vitamin K cycle in mammalian cells
We describe a cell-based assay for studying vitamin K–cycle enzymes. A reporter protein consisting of the gla domain of factor IX (amino acids 1-46) and residues 47-420 of protein C was stably expressed in HEK293 and AV12 cells. Both cell lines secrete carboxylated reporter when fed vitamin K or vit...
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| Hauptverfasser: | , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
American Society of Hematology
2011
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3062303/ https://ncbi.nlm.nih.gov/pubmed/21239697 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1182/blood-2010-08-304303 |
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