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Permanent cell lines established from ts-COS cells that regulate by temperature the amplification and expression of cloned genes.
Temperature-sensitive COS cells have been transformed at restrictive temperature with SV40 replicons containing the neo or pac markers. Puromycin-resistant cell clones maintained at the restrictive temperature contain the pac gene integrated into the cell DNA. However, when the cells are shifted to...
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| Pubblicato in: | Nucleic Acids Res |
|---|---|
| Autori principali: | , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Oxford University Press
1987
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| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC306072/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2819821/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/15.15.6117 |
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