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Permanent cell lines established from ts-COS cells that regulate by temperature the amplification and expression of cloned genes.

Temperature-sensitive COS cells have been transformed at restrictive temperature with SV40 replicons containing the neo or pac markers. Puromycin-resistant cell clones maintained at the restrictive temperature contain the pac gene integrated into the cell DNA. However, when the cells are shifted to...

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Autors principals: de la Luna, S, Portela, A, Martínez, C, Ortín, J
Format: Artigo
Idioma:Inglês
Publicat: 1987
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC306072/
https://ncbi.nlm.nih.gov/pubmed/2819821
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