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Permanent cell lines established from ts-COS cells that regulate by temperature the amplification and expression of cloned genes.
Temperature-sensitive COS cells have been transformed at restrictive temperature with SV40 replicons containing the neo or pac markers. Puromycin-resistant cell clones maintained at the restrictive temperature contain the pac gene integrated into the cell DNA. However, when the cells are shifted to...
Tallennettuna:
| Päätekijät: | , , , |
|---|---|
| Aineistotyyppi: | Artigo |
| Kieli: | Inglês |
| Julkaistu: |
1987
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| Linkit: | https://ncbi.nlm.nih.gov/pmc/articles/PMC306072/ https://ncbi.nlm.nih.gov/pubmed/2819821 |
| Tagit: |
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