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A membrane-embedded glutamate is required for ligand binding to the multidrug transporter EmrE
EmrE is an Escherichia coli multidrug transporter that confers resistance to a variety of toxins by removing them in exchange for hydrogen ions. The detergent-solubilized protein binds tetraphenylphosphonium (TPP(+)) with a K(D) of 10 nM. One mole of ligand is bound per ∼3 mol of EmrE, suggesting th...
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| Autori principali: | , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Oxford University Press
2000
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| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC305557/ https://ncbi.nlm.nih.gov/pubmed/10637227 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/emboj/19.2.234 |
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