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A membrane-embedded glutamate is required for ligand binding to the multidrug transporter EmrE
EmrE is an Escherichia coli multidrug transporter that confers resistance to a variety of toxins by removing them in exchange for hydrogen ions. The detergent-solubilized protein binds tetraphenylphosphonium (TPP(+)) with a K(D) of 10 nM. One mole of ligand is bound per ∼3 mol of EmrE, suggesting th...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2000
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC305557/ https://ncbi.nlm.nih.gov/pubmed/10637227 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/emboj/19.2.234 |
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