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Purification and Ligand Binding of EmrR, a Regulator of a Multidrug Transporter

EmrR, the repressor of the emrRAB operon of Escherichia coli, was purified to 95% homogeneity. EmrR was found to bind putative ligands of the EmrAB pump—2,4-dinitrophenol, carbonyl cyanide m-chlorophenylhydrazone, and carbonyl cyanide p-(trifluoro-methoxy)phenylhydrazone—with affinities in the micro...

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Detalhes bibliográficos
Main Authors: Brooun, Alexei, Tomashek, John J., Lewis, Kim
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 1999
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC94011/
https://ncbi.nlm.nih.gov/pubmed/10438794
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