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Purification and Ligand Binding of EmrR, a Regulator of a Multidrug Transporter

EmrR, the repressor of the emrRAB operon of Escherichia coli, was purified to 95% homogeneity. EmrR was found to bind putative ligands of the EmrAB pump—2,4-dinitrophenol, carbonyl cyanide m-chlorophenylhydrazone, and carbonyl cyanide p-(trifluoro-methoxy)phenylhydrazone—with affinities in the micro...

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Detalhes bibliográficos
Publicado no:J Bacteriol
Principais autores: Brooun, Alexei, Tomashek, John J., Lewis, Kim
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1999
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC94011/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10438794/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.181.16.5131-5133.1999
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