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Crystallization and preliminary X-ray analysis of 4-­coumarate:CoA ligase from Arabidopsis thaliana

4-Coumarate:CoA ligase 2 (4CL2) from Arabidopsis thaliana catalyzes the ATP-dependent formation of the 4-coumaroyl-CoA thioester through the formation of 4-coumarate-AMP. Recombinant 4CL2 protein was expressed in Escherichia coli and crystallized by the sitting-drop vapour-diffusion method. The crys...

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Bibliografiske detaljer
Main Authors: Morita, Hiroyuki, Mori, Takahiro, Wanibuchi, Kiyofumi, Kato, Ryohei, Sugio, Shigetoshi, Abe, Ikuro
Format: Artigo
Sprog:Inglês
Udgivet: International Union of Crystallography 2011
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3053174/
https://ncbi.nlm.nih.gov/pubmed/21393854
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309111002181
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