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Expression, purification and crystallization of a plant type III polyketide synthase that produces diarylheptanoids

Curcuminoid synthase (CUS) from Oryza sativa is a plant-specific type III polyketide synthase that catalyzes the one-pot formation of bisdemethoxy­curcumin by the condensation of two molecules of 4-coumaroyl-CoA and one molecule of malonyl-CoA. Recombinant CUS was expressed in Escherichia coli and c...

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Detalhes bibliográficos
Main Authors: Morita, Hiroyuki, Wanibuchi, Kiyofumi, Kato, Ryohei, Sugio, Shigetoshi, Abe, Ikuro
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2010
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2917300/
https://ncbi.nlm.nih.gov/pubmed/20693677
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309110023572
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