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Crystallization and preliminary crystallographic analysis of an octaketide-producing plant type III polyketide synthase

Octaketide synthase (OKS) from Aloe arborescens is a plant-specific type III polyketide synthase that produces SEK4 and SEK4b from eight molecules of malonyl-CoA. Recombinant OKS expressed in Escherichia coli was crystallized by the hanging-drop vapour-diffusion method. The crystals belonged to spac...

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Detalhes bibliográficos
Main Authors: Morita, Hiroyuki, Kondo, Shin, Kato, Ryohei, Wanibuchi, Kiyofumi, Noguchi, Hiroshi, Sugio, Shigetoshi, Abe, Ikuro, Kohno, Toshiyuki
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2007
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2339743/
https://ncbi.nlm.nih.gov/pubmed/18007047
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309107047069
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