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Crystallization and preliminary X-ray analysis of 4-coumarate:CoA ligase from Arabidopsis thaliana
4-Coumarate:CoA ligase 2 (4CL2) from Arabidopsis thaliana catalyzes the ATP-dependent formation of the 4-coumaroyl-CoA thioester through the formation of 4-coumarate-AMP. Recombinant 4CL2 protein was expressed in Escherichia coli and crystallized by the sitting-drop vapour-diffusion method. The crys...
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| 主要な著者: | , , , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
International Union of Crystallography
2011
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3053174/ https://ncbi.nlm.nih.gov/pubmed/21393854 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309111002181 |
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