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Facile Trypsin Immobilization in Polymeric Membranes for Rapid, Efficient Protein Digestion
Sequential adsorption of poly(styrene sulfonate) and trypsin in nylon membranes provides a simple, inexpensive method to create stable, microporous reactors for fast protein digestion. The high local trypsin concentration and short radial diffusion distances in membrane pores facilitate proteolysis...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3052767/ https://ncbi.nlm.nih.gov/pubmed/21087034 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac101857j |
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