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Limited Proteolysis Via Millisecond Digestions in Protease-Modified Membranes
Sequential adsorption of poly(styrene sulfonate) (PSS) and proteases in porous nylon yields enzymatic membrane reactors for limited protein digestion. Although a high local enzyme density (~30 mg/cm(3)) and small pore diameters in the membrane lead to digestion in < 1 s, the low membrane thicknes...
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| Main Authors: | , , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3683959/ https://ncbi.nlm.nih.gov/pubmed/22950601 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac3019153 |
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