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Improved protease digestion conditions for membrane protein detection
This work presents improved protease digestion conditions for membrane protein detection. The enzymatic digest of bacteriorhodopsin (BR), a model membrane protein with seven transmembrane domains (TMDs) was investigated. An initial in-gel digestion identified 17% BR sequence coverage, including part...
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| Pubblicato in: | Electrophoresis |
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| Autori principali: | , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
2015
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4739822/ https://ncbi.nlm.nih.gov/pubmed/25884272 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/elps.201400579 |
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