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Extracellular Protease Digestion to Evaluate Membrane Protein Cell Surface Localization

Membrane proteins play crucial roles in signaling and as anchors for cell surface display. Proper secretion of a membrane protein can be evaluated by its susceptibility to digestion by an extracellular protease, but this requires a crucial control to confirm membrane integrity during digestion. This...

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Bibliographic Details
Published in:Nat Protoc
Main Authors: Besingi, Richard N., Clark, Patricia L.
Format: Artigo
Language:Inglês
Published: 2015
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4838181/
https://ncbi.nlm.nih.gov/pubmed/26584447
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2015.131
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