Rapid and Efficient Protein Digestion using Trypsin Coated Magnetic Nanoparticles under Pressure Cycles

Trypsin-coated magnetic nanoparticles (EC-TR/NPs), prepared via a simple multilayer random crosslinking of the trypsin molecules onto magnetic nanoparticles, were highly stable and could be easily captured using a magnet after the digestion was complete. EC-TR/NPs showed a negligible loss of trypsin...

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Detalhes bibliográficos
Main Authors: Lee, Byoungsoo, Lopez-Ferrer, Daniel, Kim, Byoung Chan, Na, Hyon Bin, Park, Yong Il, Weitz, Karl K., Warner, Marvin G., Hyeon, Taeghwan, Lee, Sang-Won, Smith, Richard D., Kim, Jungbae
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3043614/
https://ncbi.nlm.nih.gov/pubmed/21204257
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pmic.201000378
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