Highly stable trypsin-aggregate coatings on polymer nanofibers for repeated protein digestion

A stable and robust trypsin-based biocatalytic system was developed and demonstrated for proteomic applications. The system utilizes polymer nanofibers coated with trypsin aggregates for immobilized protease digestions. After covalently attaching an initial layer of trypsin to the polymer nanofibers...

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Bibliografiske detaljer
Main Authors: Kim, Byoung Chan, Lopez-Ferrer, Daniel, Lee, Sang-Mok, Ahn, Hye-Kyung, Nair, Sujith, Kim, Seong H., Kim, Beom Soo, Petritis, Konstantinos, Camp, David G., Grate, Jay W., Smith, Richard D., Koo, Yoon-Mo, Gu, Man Bock, Kim, Jungbae
Format: Artigo
Sprog:Inglês
Udgivet: 2009
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2779140/
https://ncbi.nlm.nih.gov/pubmed/19288524
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pmic.200800591
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