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Highly stable trypsin-aggregate coatings on polymer nanofibers for repeated protein digestion
A stable and robust trypsin-based biocatalytic system was developed and demonstrated for proteomic applications. The system utilizes polymer nanofibers coated with trypsin aggregates for immobilized protease digestions. After covalently attaching an initial layer of trypsin to the polymer nanofibers...
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| Main Authors: | , , , , , , , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2779140/ https://ncbi.nlm.nih.gov/pubmed/19288524 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pmic.200800591 |
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