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Highly stable trypsin-aggregate coatings on polymer nanofibers for repeated protein digestion

A stable and robust trypsin-based biocatalytic system was developed and demonstrated for proteomic applications. The system utilizes polymer nanofibers coated with trypsin aggregates for immobilized protease digestions. After covalently attaching an initial layer of trypsin to the polymer nanofibers...

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מידע ביבליוגרפי
Main Authors: Kim, Byoung Chan, Lopez-Ferrer, Daniel, Lee, Sang-Mok, Ahn, Hye-Kyung, Nair, Sujith, Kim, Seong H., Kim, Beom Soo, Petritis, Konstantinos, Camp, David G., Grate, Jay W., Smith, Richard D., Koo, Yoon-Mo, Gu, Man Bock, Kim, Jungbae
פורמט: Artigo
שפה:Inglês
יצא לאור: 2009
נושאים:
גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC2779140/
https://ncbi.nlm.nih.gov/pubmed/19288524
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pmic.200800591
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