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Trypsin coatings on electrospun and alcohol-dispersed polymer nanofibers for trypsin digestion column
The construction of a trypsin column for rapid and efficient protein digestion in proteomics is described. Electrospun and alcohol-dispersed polymer nanofibers were used for the fabrication of highly stable trypsin coatings, which was prepared by a two-step process of covalent attachment and enzyme...
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| Main Authors: | , , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2950708/ https://ncbi.nlm.nih.gov/pubmed/20718428 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac101633e |
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