Trypsin coatings on electrospun and alcohol-dispersed polymer nanofibers for trypsin digestion column

The construction of a trypsin column for rapid and efficient protein digestion in proteomics is described. Electrospun and alcohol-dispersed polymer nanofibers were used for the fabrication of highly stable trypsin coatings, which was prepared by a two-step process of covalent attachment and enzyme...

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Main Authors: Jun, Seung-Hyun, Chang, Mun Seock, Kim, Byoung Chan, An, Hyo Jin, Lopez-Ferrer, Daniel, Zhao, Rui, Smith, Richard D., Lee, Sang-Won, Kim, Jungbae
Format: Artigo
Sprog:Inglês
Udgivet: 2010
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2950708/
https://ncbi.nlm.nih.gov/pubmed/20718428
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac101633e
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