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A rapid assay for assessment of sphingosine kinase inhibitors and substrates

Sphingosine kinases catalyze the transfer of phosphate from ATP to sphingosine to generate sphingosine 1-phosphate, an important bioactive lipid molecule that mediates a diverse range of cell signaling processes. The conventional assay of sphingosine kinase enzymatic activity uses [γ-(32)P]ATP and s...

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Bibliografiske detaljer
Main Authors: Kharel, Yugesh, Mathews, Thomas P., Kennedy, Andrew J., Houck, Joseph D., Macdonald, Timohy L., Lynch, Kevin R.
Format: Artigo
Sprog:Inglês
Udgivet: 2011
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3049835/
https://ncbi.nlm.nih.gov/pubmed/21216217
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2011.01.003
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