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A rapid assay for assessment of sphingosine kinase inhibitors and substrates
Sphingosine kinases catalyze the transfer of phosphate from ATP to sphingosine to generate sphingosine 1-phosphate, an important bioactive lipid molecule that mediates a diverse range of cell signaling processes. The conventional assay of sphingosine kinase enzymatic activity uses [γ-(32)P]ATP and s...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3049835/ https://ncbi.nlm.nih.gov/pubmed/21216217 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2011.01.003 |
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