Rapid, low-input, low-bias construction of shotgun fragment libraries by high-density in vitro transposition

We characterize and extend a highly efficient method for constructing shotgun fragment libraries in which transposase catalyzes in vitro DNA fragmentation and adaptor incorporation simultaneously. We apply this method to sequencing a human genome and find that coverage biases are comparable to those...

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Bibliografiske detaljer
Main Authors: Adey, Andrew, Morrison, Hilary G, Asan, Xun, Xu, Kitzman, Jacob O, Turner, Emily H, Stackhouse, Bethany, MacKenzie, Alexandra P, Caruccio, Nicholas C, Zhang, Xiuqing, Shendure, Jay
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2010
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Method
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3046479/
https://ncbi.nlm.nih.gov/pubmed/21143862
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/gb-2010-11-12-r119
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