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DNA Library Preparation: Simultaneous DNA Fragmentation and Adaptor Tagging by In Vitro Transposition

RP-69 DNA library preparation is a common entry point and bottleneck for next-generation sequencing. Current methods generally consist of distinct DNA fragmentation, end-polishing, and adaptor-ligation steps; often with significant sample loss and hands-on time. EPICENTRE Biotechnologies has develop...

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Detalhes bibliográficos
Main Authors: Caruccio, N., Grunenwald, H., Begum, D., Maffitt, M., Wang, Y.
Formato: Artigo
Idioma:Inglês
Publicado em: Association of Biomolecular Resource Facilities 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2918206/
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