Directed Evolution of DNA Polymerases for Next Generation Sequencing

[Image: see text] We present the application of an activity-based phage display method to identify DNA polymerases tailored for next generation sequencing applications. Using this approach, we identify a mutant of Taq DNA polymerase that incorporates the fluorophore-labeled dA, dT, dC, and dG substr...

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Auteurs principaux: Leconte, Aaron M., Patel, Maha P., Sass, Lauryn E., McInerney, Peter, Jarosz, Mirna, Kung, Li, Bowers, Jayson L., Buzby, Philip R., Efcavitch, J. William, Romesberg, Floyd E.
Format: Artigo
Langue:Inglês
Publié: 2010
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Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3043640/
https://ncbi.nlm.nih.gov/pubmed/20629059
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201001607
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